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MEDIA OPTIMIZATION FOR LIPASE FROM BACILLUS LICHENIFORMIS, MODERATE HALOPHILES ISOLATED FROM EXCRETA OF WILD ASS. Khunt MD , Pandhi ND

MEDIA OPTIMIZATION FOR LIPASE FROM BACILLUS LICHENIFORMIS, MODERATE HALOPHILES ISOLATED FROM EXCRETA OF WILD ASS.

Khunt MD , Pandhi ND

International Journal of Applied Microbiology Science 2012; 1; 8-14.

Abstract

Twenty-four moderate halophiles were isolated from excreta of wild ass after enrichment. All the isolates were screened for extracellular lipase secretion on solid media containing tributyrin as carbon source. B.licheniformis was identified on the basis of 16s r-RNA sequencing and on the basis of zone ratio, further optimized for lipase secretion in liquid media. Maximum lipase secretion was after 264 hours of incubation i.e. during stationary phase. Isolates were found to secrete maximum lipase at pH 5-6, 10%-13% NaCl, 40˚C temperature and 6% substrate concentration.

Keywords: Lipases, moderate halophiles, Rann of Kutch, wild ass.
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