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CYTOTOXICITY AND WOUND HEALING PROPERTIES OF COPAIFERA LANGSDORFFII OLEORESIN IN RABBITS. Masson-Meyers DS, Andrade TAM, Leite SN, Frade MAC

CYTOTOXICITY AND WOUND HEALING PROPERTIES OF COPAIFERA LANGSDORFFII OLEORESIN IN RABBITS

Masson-Meyers DS, Andrade TAM, Leite SN, Frade MAC

International Journal of Natural Product Science 20 13; 3(3):10-20

Abstract

Plants of the Copaifera genus (copaiba) are documented to have healing potential; however, their cytotoxicity and therapeutic mechanisms remain unclear. This study assessed in vitro cytotoxicity of copaiba oleoresin from Copaifera langsdorffii on 3T3 fibroblasts and investigated its wound healing potential in rabbits. Cell viability and proliferation were assessed by MTT and H3-Thymidine assays, respectively. Wounds were created on rabbits’ ears and topically treated with saline (S), control cream (Cr), 10% (C10) and 25% (C25) copaiba cream, or pure oleoresin (OR) over 21 days, and assessed 2, 7, 14 and 21 days post-wounding by wound healing rates and histology. Fibroblasts treated with up to 100 µg/mL of copaiba yielded greater than 80% of viability and proliferation compared to control culture, decreasing significantly (p<0.001) at 500 and 1000 µg/mL. Wounds were re-epithelialized by the 21st day, with some OR-treated wounds showing evidence of sustained inflammation on day 7. C10 and C25-treated wounds presented better clinical outcomes, confirmed by histology with evidence of fibroblastic activity by day 7 and organized collagen fibers observed from day 14. These findings suggest that C. langsdorffii oleoresin shows no cytotoxic effects on fibroblasts at 100 µg/mL and at appropriate doses enhances wound healing.


Keywords: Copaifera langsdorffii, cytotoxicity, efficacy, wound healing
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