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ANTIMICROBIAL PROPERTIES AND PHYTOCHEMICAL PROFILING OF ESSENTIAL OILS EXTRACTED FROM TRADITIONALLY USED MEDICINAL PLANTS IN BENIN. Baba-Moussa F, Adjanohoun A, Adéoti K, Gbénou JD, Aloukoutou D, Kpavodé L, Moudachirou M, Akpagana K, Bouchet P, Kotchoni SO, Toukourou F, Baba-Moussa L

ANTIMICROBIAL PROPERTIES AND PHYTOCHEMICAL PROFILING OF ESSENTIAL OILS EXTRACTED FROM TRADITIONALLY USED MEDICINAL PLANTS IN BENIN. Baba-Moussa

F, Adjanohoun A, Adéoti K, Gbénou JD, Aloukoutou D, Kpavodé L, Moudachirou M, Akpagana K, Bouchet P, Kotchoni SO, Toukourou F, Baba-Moussa L

International Journal of Natural Product Science 2012; 2(4): 1-11

Abstract

Essential oils of different medicinal plants including Clausena anisata, Cymbopogon citratus, Cymbopogon nardus, Eucalyptus camaldulensis, Eucalyptus citriodora, Lantana camara, Lippia multiflora, Melaleuca quinquenervia, Mentha piperita, Ocimum basilicum, Ocimum gratissimum, Citrus aurantium and Xylopia aethiopica were extracted and tested in this work for their antimicrobial properties against various human infectious pathogenic agents. The essential oils were tested against 5 pathogenic strains (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans) using the agar diffusion and microdilution methods. Ocimum gratissimum essential oil was found to have the strongest antimicrobial effect with the inhibition diameter of 31±2 mm on Staphylococcus aureus, while Lippia multiflora essential oil was found to have the least antimicrobial effect (inhibition diameter of 10 ± 2 mm) on Enterococcus faecalis. The Minimal Inhibitory Concentration (MIC) varies from 0.156 mg/mL to 10 mg/mL depending on the essential oil. Our results showed that Ocimum gratissimum have the lowest MIC (0.156 to 2.5 mg/mL) as expected. The chromatographic derived phytochemical profiling analysis showed thymol, 1,8-cineole, sabinene, citral, limonene and estragole to be the major components of the essential oils.

Keywords: Antimicrobial activity, Benin, essential oils, medicinal plants.
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