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STUDY ON LUFFA ECHINATA SEEDS FOR ANTIOXIDANT POTENTIAL BY USING HYDROGEN PEROXIDE ASSAY. Tanvi Sharma, Rashmi Arora, N.S.Gill

STUDY ON LUFFA ECHINATA SEEDS FOR ANTIOXIDANT POTENTIAL BY USING HYDROGEN PEROXIDE ASSAY.

Tanvi Sharma, Rashmi Arora, N.S.Gill

International Journal of Natural Product Science 2012: Spl Issue 1:227.

Abstract(RBIP-227)

The objective of the present study was to evaluate the in-vitro antioxidant activity of methanolic extract of Luffa echinata seeds by using H2O2 free radical scavenging assay. Preliminary phytochemical screening was done to find the major constituent in selected plant. A solution of H2O2 (43mM) was prepared in 0.1M phosphate buffer (pH 7.4).Different concentrations (50-150µg/ml) of standard and extract in distilled water were added to H2O2 solution(0.6ml) andconcentration of H2O2 was determined by taking absorbance at 230nm against a blank solution.Ascorbic acid was used as standard drug. Phytochemical tests were performed for alkaloids, flavonoids, glycosoides, tri-terpenoids, sterols, carbohydrates and for proteins. Antioxidant effect of plant is also investigated qualitatively by using DPPH(1,1-Diphenyl-2-picrylhydrazyle)radical scavenging method. The extract will be further evaluated for its anti-inflammatory and analgesic activities.

Maximum free radical scavenging activity of crude methanolic extract of Luffa echinata was shown at dose 150 μg/ml is 69.16±0.94by H2O2 model. The IC50 of the extract was found to be ≤50µg.Phytochemical evaluation of methanolic seed extract of Luffaechinata showed the presence of flavonoids, alkaloids, triterpenoides compounds. From literature survey it was found that flavonoids are mainly responsible for antioxidant activity.The result suggested that methanolic extract of Luffa echinata seeds possessed significant antioxidant activity.
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