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IN-VITRO EVALUATION OF ANTIOXIDANT AND MEMBRANE STABILIZATION CAPACITIES OF JUGLAS REGIA USING MULTIPLE ANTIOXIDANT ASSAYS. Deepinderjeet Singh Joshan, Shruti Rawal

IN-VITRO EVALUATION OF ANTIOXIDANT AND MEMBRANE STABILIZATION CAPACITIES OF JUGLAS REGIA USING MULTIPLE ANTIOXIDANT ASSAYS.

Deepinderjeet Singh Joshan, Shruti Rawal

International Journal of Natural Product Science 2012: Spl Issue 1:165.

Abstract(RBIP-165)

Reactive oxygen species (ROS) are thought to underline the process of ageing and the pathogenicity of various diseases, such as neurodegenerative disorders and cancer. The use of traditional medicine is widespread and plants still present a large source of natural antioxidants that might serve as leads for the development of novel drugs. This study was aimed at evaluating the antioxidant and membrane stabilizing activities of 70% ethanolic extract of Juglans regia kernels family Juglandaceae. Juglans regia has been assayed by using in vitro methods like 2, 2-diphenyl-1-picrylhydrazylhydrate (DPPH) assay, reductive ability, and superoxide anion scavenging activity, total polyphenolic and total flavonoid content, in order to explain the role of antioxidant principles of the extract. Total flavonoids and polyphenolics were found to be 0.020±0.289 mg Quercetin equivalents (QE)/g, 0.009±0.385 mg gallotannic acid (GAE)/g and 0.138±0.261 GAE/g of dry extract respectively. The antioxidant capacity of sample was assessed through DPPH-scavenging, superoxide scavenging and metal chelating assay (with IC50 values of extract being 32.84µg/ml, 6.9µg/ml and 3.51µg/ml) compared to reference antioxidant compounds. The anti-inflammatory activity was carried out by in vitro human red blood cell (HRBC) membrane stabilization method involving response observation of the extract (61.20±0.73%) compared to diclofenac sodium (77.04±0.91%) at 600µg/ml. Also, these various antioxidant activities were compared to BHA, ascorbic acid, quercetin and BHT as reference antioxidant compounds.

Keywords: Juglans regia, ROS, antioxidant, membrane stabilization, kernels, IC50 value.
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