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RECENT ANALYTICAL METHODS FOR THE DETERMINATION OF DERMATOPHARMACOKINETICS. Vijender kumar, Srikanth Pandray, Sunil Kamboj, Suman Bala, Vipin Saini

RECENT ANALYTICAL METHODS FOR THE DETERMINATION OF DERMATOPHARMACOKINETICS.

Vijender kumar, Srikanth Pandray, Sunil Kamboj, Suman Bala, Vipin Saini

International Journal of Natural Product Science 2012: Spl Issue 1:73.


Abstract (RBIP-73)

Dermatopharmacokinetics is the penetration kinetics of drugs and cosmetic products in or through the skin. It has significant influence in the action and the protection properties of topically applied substances. The determination of the penetration kinetics and the penetration pathways of chemical compounds is still an object of intensive investigations. Different analytical in vitro and in vivo methods were used this purpose. In vitro methods include Franz cell experiments, Biopsy, Tape stripping etc. In vivo methods include radioactive labelling, Biopsy, Tape stripping, Surface recovery, Biological/pharmaceutical response, Autoradiography, Fluorescence measurements etc. It is sometimes difficult to correlate the in vitro to the in vivo measurements. The in-vitro measurements have the advantage that both allergens and toxic substances can be investigated. The first three in vivo methods allow both a qualitative analysis of the penetration of topically applied substances into the skin. The time factor must be taken into consideration for the determination of the penetration kinetics. This means that the process must be analyzed either by on-line measurements or by taking samples from the same volunteer at different times. Although well-suited to penetration measurements, the method of applying radioactive labelled substances is unacceptable from an ethical point of view. This applies also to skin biopsies. Tape stripping is a non-invasive method only suited to analyze the stratum corneum. A new analytical method for penetration measurements was developed at the Center of Experimental and Applied Cutaneous Physiology (CCP). The method is based on the well-known tape stripping technique in combination with spectroscopic measurements that allow the determination of the amount of corneocytes on each tape strip removed.
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