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SCREENING AND CHARACTERIZATION OF LIPASE ENZYME ISOLATED FROM OILY SOIL Singh Sanpreet, Joshi Mahavir, Gupta Mansi

SCREENING AND CHARACTERIZATION OF LIPASE ENZYME ISOLATED FROM OILY SOIL.

Singh Sanpreet, Joshi Mahavir, Gupta Mansi

International Journal of Biochemistry & Biotech Sciences 2013; 2(2):1-7

Abstract

Enzymes are well known for their catalytic activities. Further it is seen that there are different sources of enzyme secretion and synthesis viz., bacteria, fungi, yeast, plant etc. Basically lipase enzyme has been used for industrial and research purposes because of its low cost and high stability. Lipase can be isolated from bacteria residing in oily soils. The isolated bacteria were identified and these belongs to Staphylococcus class. Further screening was done by using tributyrin as substrate in nutrient agar media. Biochemical characterization along with motility tests were also performed for its identification. Statistical optimization of media parameters was done to analyze the enzyme activity in which effect of pH, incubation period, temperature, agitation speed and carbon and nitrogen source in form of oil and extract was carried out. It was observed that all the above said parameters affect the enzymatic activity of this lipolytic enzyme i.e lipase.

Keywords: Lipase, motility test, staphylococcus, tributyrin
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