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SCREENING OF PECTINASE PRODUCING BACTERIA AND FUNGI FOR ITS PECTINOLYTIC ACTIVITY USING FRUIT WASTES. M. Geetha, P.Saranraj, S. Mahalakshmi and D. Reetha

SCREENING OF PECTINASE PRODUCING BACTERIA AND FUNGI FOR ITS PECTINOLYTIC ACTIVITY USING FRUIT WASTES.

M. Geetha, P.Saranraj, S. Mahalakshmi and D. Reetha

International Journal of Biochemistry & Biotech Science 2012; 1: 30-42

Abstract

Fruit peel wastes, one of the polluting solid wastes has been utilized for the production of pectinase enzymes by solid state and submerged fermentation employing pectinolytic microorganism isolated from fruit peel wastes. Pectinolytic bacterial and fungal cultures were isolated from fruit peel wastes. The bacterial cultures were identified as Bacillus sp. and Pseudomonas sp. and the fungal cultures were identified as Aspergillus niger, Aspergillus flavus and Penicillium chrysogenum. All the bacterial and fungal cultures were found to produce appreciable level of pectinolytic enzyme viz., pectin esterase and pectate lyase. Among the different levels of pectin tested, all the cultures recorded maximum pectinase activity at one percent pectin concentration. Maximum pectate lyase and pectin esterase activity was expressed by Bacillus sp. grown in medium with one percent pectin concentration. All the fungal isolates showed maximum activity of pectin esterase and pectate lyase activity. Among the fungal isolates, Aspergillus niger recorded maximum pectin esterase and pectate lyase activity. Under submerged fermentation, the fungal isolate Aspergillus flavus recorded higher pectin esterase and pectate lyase. The Pectinase production from fruit peel was higher in solid state fermentation than in submerged fermentation and Aspergillus performed better than other organisms.

Keywords: Fruit wastes, Pectinase, Bacteria, Fungi, Pectin esterase, Pectate lyase and Fermentation.
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