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THE ANTI-HYPERGLYCEMIC ACTIVITY OF PARTIALLY PURIFIED EXTRACT FROM THE ROOTS OF THOTTEA GRANDIFLORA ROTTB. Okechukwu NP

THE ANTI-HYPERGLYCEMIC ACTIVITY OF PARTIALLY PURIFIED EXTRACT FROM THE ROOTS OF THOTTEA GRANDIFLORA ROTTB.

Okechukwu NP

International Journal of Pharmacology & Toxicology Science 2011; 3: 22-31

Abstract

Thottea grandiflora Rottb, locally known as ‘hempedu beruang’ is used as a traditional remedy to treat diabetes and complications of this disease. Preliminary pharmacological investigation revealed that the dichloromethane (DCM) crude root extracts possessed anti-diabetic activity. In the present study Fraction A-E obtained from the partial purification of DCM TG root extract and further purified sub-fractions E1, E2 and E3 were evaluated for their antidiabetic effects using fifty five STZ-induced wistar strain albino male rats (6-8weeks), weighing 200-210g for 4 weeks. The blood glucose levels and the bodyweights of the diabetic rats were determined before and at week 0 and week 4 of treatment. Fraction E and sub-fraction E1 significantly (p < 0.05) reduced the blood glucose levels of the diabetic rats by an average of 32.13% and 44.63% increased the bodyweights of the diabetic rats by an average of 8.69% and 8.12 % respectively which correlates with positive control tolbutamide. Fraction A-D, sub-fraction E2 & E3 did not show any positive effects. Lactate dehydrogenase enzyme (LDH) activity was found to be elevated in the group treated with fraction E (2.686 U/g) and sub fraction E1 (2.931 U/g) similar to reference drug tolbutamide. Phytochemical tests of fraction E and sub-fraction E1 revealed the presence of alkaloids, flavonoids, saponins and terpenoids. HPLC profiling of sub-fraction E1 show the presences of Quercetin, Kaempherol, Catechin, Caffeic acid, Berberine most of these phytochemicals have been reported to possess anti-diabetic activity. Hence, the anti-diabetic effects of fraction E and sub-fraction E1 could be due to the presence of these phytochemicals.

Keywords: Dichloromethane, antidiabetic, hyperglycemia, phytochemicals, Thottea grandiflora Rottb
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